منابع مشابه
Hela Cell Plasma Membranes
A method for the preparation of HeLa cell plasma membrane ghosts is described. The purity of the plasma membrane fraction was examined by phase contrast and electron microscopy, by chemical analysis, and by assay of marker enzymes. Data on the composition of the plasma membrane fraction are given. It was observed that the distribution pattern of 5'-nucleotidase activity among the subcellular fr...
متن کاملPurification and properties of HeLa cell plasma membranes.
A simple method for the preparation of HeLa cell plasma membrane ghosts without the use of cross-linking reagents or Cxatives has been developed. The membranes have been characterized by phase and electron microscopy and by the use of radioactive precursors to RNA, protein, glycoprotein, and lipid. The glycoprotein and polypeptide constituents have been analyzed by sodium dodecyl sulfate-acryla...
متن کاملPlasma lipids, lipoproteins and cell membranes.
The major plasma lipids, cholesteryl ester, triacylglycerol, cholesterol and phospholipid, are insoluble in water. They are transported in the circulation as lipoproteins-metabolically diverse macromolecules in which the various lipids are associated with specific polypeptides (the apoproteins). There are four main lipoprotein classes, defined by their size and by the density range within which...
متن کاملThe binding of plasma proteins to human placental cell membranes.
1. To investigate the relative degree. to which human IgG and other plasma proteins bind to cell membranes of the full-term human placenta, suspensions of membranes mixed with radio-iodinated proteins were incubated at pH 6.5 and subjected to Sepharose 2B column chromatography. The amount of labelled protein associated with membrane protein in membrane-containing fractions of the eluate was the...
متن کاملCationic nanoparticles induce nanoscale disruption in living cell plasma membranes.
It has long been recognized that cationic nanoparticles induce cell membrane permeability. Recently, it has been found that cationic nanoparticles induce the formation and/or growth of nanoscale holes in supported lipid bilayers. In this paper, we show that noncytotoxic concentrations of cationic nanoparticles induce 30-2000 pA currents in 293A (human embryonic kidney) and KB (human epidermoid ...
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ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 1974
ISSN: 1540-8140,0021-9525
DOI: 10.1083/jcb.63.2.357